This paper develops the energy saving light source for purple non-sulfur photosynthetic bacteria growth at room temperature in the Microbiology Laboratory, Khon Kaen Univesity (KKU), Thailand. The non-sulfur photosynthesis bacteria, Rhodopseudomonas sp., is an emerging technology to generate hydrogen, the efficient energy carrier.  As light source, LED consumes less power than fluorescent. Two type of the 1W high power LEDs, the warm white and cool white, were assembled for higher illumination. The high efficient light sources are able to reduce energy compared to that of fluorescent by 50.8% for the 10-day growing interval of the SN-1/1 strain. LEDs can be recommended as light source for purple non-sulfur photosynthetic bacteria growth.