Burapha University International Conference, BUU-2014

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Environmental Effects on In vitro Bovine Embryo Production
Ashit Kumar Paul

Last modified: 2014-05-25

Abstract


Global warming is the time claimed burning issue around the world. Animals live with very specific climate conditions that enable them to thrive. Any change in the climate can affect the animal’s normal body physiology. Oocytes quality is a key factor affecting on in vitro embryo production (IVEP). In this study, we hypothesized that the environment has the strong relationship for in vitro embryo development. Therefore the present study was designed to explore the environmental effect and its strength of association on IVEP. In this research, we recorded the temperature, humidity and rainfall at day of oocytes collection to find out their effect on oocytes quality and embryonic development. Following that we fertilized the both dairy and beef oocytes with three different bull semen and cultured to calculate the cleavage and blastocyst rate. From our partial study, we found that the environmental factors i.e. temperature, humidity and rainfall, season affect the IVEP. The achievement of good quality oocytes is markedly varied in different environmental factors. The quality of oocytes is very crucial for its further in vitro maturation, fertilization and embryonic development. The high rainfall and temperature at the day of oocytes collection decrease the IVEP rate whereas the winter season showed higher blastocyst rate than that of others. The breeds of cows and different bull semen also have the significant (p<0.05) effect on IVEP. After completed this project we might find out the environmental relationship with in vitro embryo production.