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This research work aimed to optimize the oil extraction condition of Jatropha kernel. The experiment was divided into two parts, finding the best size of the kernel for extraction and using statistical design to find the condition of the maximum % extracted oil yield. For the first experiment, Jatropha kernel was grinded into size 250-425 microns and extracted at the fixed condition. The best size was chosen from the minimum remain oil left at the kernel. In the second experiment, three-factor-three-level Box-Behnken response surface methodology was applied to design the experimental runs and find the optimum condition for oil extraction of the kernel at size between 0.425 and 0.250 mm. Commercial hexane was used in this experiment and there were 3 parameters namely reaction time (30-150 minutes), ratio of hexane to Jatropha kernel (10:1-60:1 ml/g), and agitation rate (100-500 rpm). Then the model of the optimum condition was suggested by the Minitab program which was 84 minutes, 27 ml/g and 313 rpm. Finally, the experiment at the suggested optimum condition was performed and the result was compared with the model. It was found that the result from the experiment fit well with the model prediction with the error of 4.48 %.