The objective of the present study was to formulate and evaluate the physicochemical properties and stability of liposomal vesicles consisting of crude protein extract derived from Pigeon Pea (Cajanus Cajan (L.) Millsp). The samples of liposomes were prepared by the thin film hydration method using rotary evaporator. The stability of liposomes was performed at 4 ⁰C for 6 months of storage. The physicochemical properties of liposomes were characterized on sizes, percentage of encapsulation efficiency (% EE), morphology, pH and color. The safety evaluations of crude protein extract loaded liposomes were then evaluated on the cytotoxicity test using MTT assay and acute skin irritation test. The results demonstrated that the crude protein extract could be loaded into the liposomal vesicles, white suspension of colloids was found. The % EE of crude protein extract loaded liposomal vesicles was found to be in the range of 60.0 - 70.0 %. The particle sizes of liposomes were in the range of micrometer (6.0 -7.0 µm). The formulation of liposomes showed no toxicity in normal skin fibroblast cell and no skin irritation in rabbit skin.  The liposomes exhibited the good stability during storage at 4 ⁰C for 6 months. It slightly declined the % EE from 70.0 % to be 60.0 % and was related to no change in morphology after storage. Therefore, crude protein extract derived from Pigeon Pea loaded liposomes exhibited the good formulation, high stability and no toxicity, contributing to high efficacy for applied to the cosmetic delivery.