Abstract 

    For growth and nutrition were studies.  Marine microalgae, Tetraselmis glacilis were cultured with six values of salinity. Microalgae were grown in 2L glass bottles containing Guillard “f/2” medium with salinity values at 25, 30, 35, 40, 45 and 50 ppt and fed under a 12 h light: 12 h dark regime of illumination using a lighting system that delivered 4000 Lux. In each experiment of microalgae, the early stationary phase was used analyzed for total lipid, total protein and fatty acid profile.

    The results found that T. glacilis cultured with salinity value at 35 ppt showed highest protein. Lipid content in T. glacilis cultured with salinity value at 35 ppt was higher than other experiments. The profile of fatty acid in T. glacilis cultured with salinity value at 35 ppt showed the highest polyunsaturated fatty acid among T. glacilis cultured with other experiments. The characteristic of the fatty acid profile of T. glacilis was a high amount (17.93-19.77 % of total fatty acid) of C16:0, C18:3n3 (14.66-16.37 % of total fatty acid) and C18:1n9 (10.73-14.86 % of total fatty acid), respectively. Each experiment showed high accumulated 18-carbon fatty acids. However, T. glacilis cultured with salinity value at 45 ppt represented a high amount (3.42 % of total fatty acid) of EPA (C20:5n3) than other experiments. In general different concentration salinity in culture medium of T. glacilis affected density of high cell. T. glacilis growth was higher when cultured with salinity values at 30 and 35 ppt than other experiments.

Keywords: nutrition, fatty acid, protein, lipid, microalgae