Actinomycetes are a group of prokaryotic organisms belonging to Gram-positive bacteria and play an important ecological role in recycling substances in the nature. The objectives of this study were isolation and screening on endophytic actinomycetes from sugar cane against phytopathogenic fungus of sugar cane red rot diseases. The results showed that 32 endophytic actinomycetes were isolated from sugar cane. Among these, 25, 5 and 2 were isolated from plant parts; root, leave and stem, respectively. There are 7 isolates from those showed significant inhibition on the growth of Sclerotium rolfsii whereas 22 isolates showed less inhibition of the fungus. Furthermore, 3 isolates has no inhibition activities. Seven isolates (SCR18, SCR17, SCR1, SCR2,SCR3, SCS4, SCR21) with high inhibition activities were selected and grouped in to 5 groups based on morphological characters and type of DAP isomers. Among these 6 isolates from 4 groups (Gr1, Gr2, Gr3, G5) were assigned as genus Streptomyces and one group (Gr4) was found belonging to member of genus Micromonospora