Diacylglycerol kinase (DGK) phosphorylates diacylglycerol (DAG) to produce phosphatidic acid (PA) and regulates PKC activity through attenuation of DAG in the lipid signaling. In this study, DGKβ, γ, ζ isozymes were localized in the kidney of adult normal and restraint-stressed mice. Intense immunoreactivities for the three isozymes were distributed in the cortex and medullary papilla of both groups, but their subcellular localization was different. For DGKβ, positive immune-reactions were found in the cytoplasm of the proximal tubules, but not Henle’s loops, distal tubules or collecting ducts of both groups. For DGKγ, intense immune-reactions were found in the epithelium cytoplasm of the parietal layers of Bowman’s capsules and proximal tubules, but not Henle’s loops, distal tubules and collecting ducts. Furthermore, immune-positive globules were found in the Bowman’s space. For DGKζ, the immunoreactivity for was detected in epithelial nuclei of Henle’s loops, collecting duct and intraglomerular cells as well as nuclei of capillary endothelial cells and interstitial cells. In addition to the nuclei, positive immunoreaction was seen in the cytoplasm of the proximal tubules, distal tubules, papillary ducts and parietal epithelial cells of Bowman’s capsule. These data suggest that each DKG isoform has a spatial heterogeneity along the uriniferous tubular system in term of the involvement in the intraepithelial lipid signaling in the kidney.