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The effect of temperature on Marine Bacterial Numbers in seawater of sampling sites located at Pattaya Beach area: Na Klua market site, North Pattaya beach site, Southern Pattaya beach site, and Na Jomtian beach site of Chon Buri Province, Thailand were determined during March to December, 2011. The results showed that marine bacterial numbers in seawater were in a range of (3.60 ± 1.65) x10² to (9.83 ± 1.50) x10³ CFU/mL. The relationship of temperature on marine bacteria was investigated. Seawater temperature of all sampling sites at Pattaya Beach fluctuated in ranges of 26.2 ± 0.3 to 34.9 ± 0.0 °C. Temperature fluctuation in seawater was no correlated with marine bacterial numbers in all sampling sites. For marine bacterial diversity found in seawaters classified to 4 groups including Enterobacteriaceae, Pseudomonadaceae, Vibrionaceae and Bacillus. Moreover, the human pathogenic bacteria were identified to Klebsiella pneumoniae, V. parahaemolyticus, while V. owensii, V. sinaloensis and V. harveyi as well as V. parahaemolyticus were aquatic animal pathogen found in the present study.