Burapha University International Conference, BUU-2014

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In vitro induction of mycelial growth in Malassezia species
Weerapong Juntachai

Last modified: 2014-06-11

Abstract


The yeasts in the genus Malassezia are known as a dimorphic microflora normally found on skins of both human and animals. Theses yeasts are also an opportunistic pathogen associated with various skin diseases such as pityriasis versicolor, and seborrheic dermatitis, and in rare cases systemic infection in neonates. The cells with mycelial form are frequently observed from the patient specimens and though to be involved in their pathogenicity. However, the mechanism of morphological change of Malassezia and its role in pathogenicity has been poorly understood due to the fastidiousness in growth of the fungi and a lack of appropriate protocol to produce mycelial phase in vitro. Solid and liquid induction medium (IM) successfully induced mycelial growth of M. furfur in vitro but was unable to induce morphological change in other Malassezia species. In addition, mycelial growth did not possess ability of invasive growth in M.furfur in vitro.