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The seasonal colonization pattern of lignicolous fungi on bait woods in shrimp ponds were conducted in Chanthaburi Province. Three shrimp ponds were selected as the test sites over a12 month period extending from January 2010 to December 2011. Every 3 months, one batch of bait woods from each pond (15 wood baits) was retrieved for one month incubation prior to evaluate of diversity. The fungi were identified and isolated into axenic cultures. The total number of fungi recorded was 18 species. The highest numbers of fungal species found were 16 species from the April 2010 sample, which comprised 13 Ascomycota and 3 anamorphic fungi. Four fungi were commonly encountered (frequency of occurrence >25%),

Periconia prolifica, Lulworthia grandispora, Antennospora quadricornuta and Verruculina enalia. Four species of Marinosphaera mangrovei, Corollospora colossa, Corollospora bulbosa, Torpedospora sp. and Monodictys pelagica can be considered as rare fungi (frequency of occurrence <2%). Shannon-Wiener index (H') for the fungi was determined to be in the range 1.44-2.25, whilst evenness (J') value was close to 1 (0.66-0.83) suggesting that the major fungal species that were found were regularly encountered and present in high abundance. Keywords: Diversity, Fungi, Seasonal colonization, Shrimp pond, Thailand